FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS45mS2-N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS45mS2-N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6990668
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG96650.066.769116
TTATAGG160900.066.750946
GGTATAG171000.066.575885
CGTATAA90900.066.528665
CTTATAG90350.066.236435
CTATACG73250.065.739646
GTATAGG195950.065.633966
CCTATAC62650.065.468965
CGTATAG109300.065.382955
CCTATAG64450.064.889435
AGTATAG129300.064.634555
TTATACG97100.064.477426
GTATAAG136850.064.4680566
CTTATAC87650.064.4037865
CTATAAC68900.064.302966
TATAGGG179950.064.294037
TTATAGT158200.064.2401666
GTTATAG171050.064.1421365
GGTATAC94700.064.0808265
TATACGG105900.063.9777577
Produced by FastQC (version 0.11.5)