FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS45mS1-noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS45mS1-noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4652819
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG61050.065.1728066
GGTATAG106700.064.971225
CGTATAA52800.064.4881445
CTATACG45900.064.19356
CTTATAG60900.064.185495
CGTATAG63100.063.9996225
TTATAGG107150.063.9784776
CCTATAC36100.063.88215
GTATAGG118750.063.7403686
GGTATAC61650.063.688465
TATACGG63350.062.9736947
GCTATAC52100.062.8693665
TATAGTG132250.062.5536657
TTATAAG79400.062.4512256
TATAGGG118700.062.2650227
GTATAAG90800.062.2027136
TATAGGC81950.062.0892457
GGTATAA86250.061.87455
CTATAAC43550.061.8720056
TATAGGT113650.061.6754727
Produced by FastQC (version 0.11.5)