FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS45mS1-N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS45mS1-N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8090971
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC84070.10390594651742047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG120250.066.643556
TTATAGG196600.066.376846
GGTATAG199450.066.34285
CTATACG88250.066.223096
CGTATAA102400.066.164735
GTATAGG229250.066.143486
CTTATAG103550.066.0720445
CGTATAG130600.065.758595
CCTATAC67850.065.505085
GCTATAC101950.065.152355
AGTATAG161550.064.966675
TATACGG133400.064.770267
TATAGGC156900.064.570787
GTATAGC135700.064.49736
CTATAAC83900.064.401066
GGTATAC118000.064.387115
TATAGGG222350.064.3404547
TTATACG121650.064.2943956
GTATAAG159600.064.135826
TTATAGT178250.063.9434286
Produced by FastQC (version 0.11.5)