FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS30mS3-noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS30mS3-noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4512302
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG59100.065.492016
CGTATAA51400.065.363975
TTATAGG108350.065.308986
CTATACG43400.065.154186
GTATAGG113550.064.8762366
CTTATAG57500.064.5161365
CCTATAC37100.064.145365
GGTATAG108600.063.999965
GGTATAC58750.063.6795775
CGTATAG62450.063.493285
TATACGG62800.063.4173557
TATAGGG113800.063.1045387
GCTATAC48800.062.965845
AGTATAG77050.062.8173835
TATAGGC81300.062.5031057
GTTATAG108250.062.2024425
TTATAGC72850.062.017946
TATAGTG125750.061.8666957
GTATAAG83400.061.8098076
TATAGGT115700.061.765777
Produced by FastQC (version 0.11.5)