FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS30mS3-N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS30mS3-N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6131365
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG142450.066.552436
GGTATAG162600.066.463195
CCTATAC51200.066.438645
CTATAGG86850.066.325016
CGTATAA80600.065.954925
GTATAGG177700.065.737966
CTTATAG78500.065.6239855
CTATACG65700.065.3576666
CGTATAG102950.065.301845
GGTATAC86950.065.042345
CTTATAC73450.064.942425
TATACGG95500.064.531217
AGTATAG116450.064.43325
TATAGGG157250.064.338557
GCTATAC77100.064.092135
TTATAGT139950.064.090416
GTATAGC108300.064.013766
TATAGGC114150.063.7984287
TTATACG84900.063.643926
TATAGGT178850.063.338477
Produced by FastQC (version 0.11.5)