FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS30mS2-noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS30mS2-noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3322128
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC27400.066.9262165
CTTATAG39300.065.539155
GTATAGG83450.065.084026
CGTATAA39750.065.0613255
CGTATAG44650.064.975355
GGTATAG75000.064.9054955
CTATAGG48050.064.892336
CTATACG30200.064.5442056
TTATAGG74850.064.146266
GTATAGC50250.064.070896
GGTATAA59400.063.7465025
GGTATAC43150.063.6655435
GCTATAC37400.063.1608125
TATACGG49350.062.9001967
TATAGTG89100.062.8038567
CTATAAC31800.062.727326
AGTATAG63500.062.7168275
TATAGGC60450.062.697147
TATAGGG86350.062.6965337
GTTATAA57300.062.5404325
Produced by FastQC (version 0.11.5)