FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS30mS2-N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS30mS2-N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5500450
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG57850.066.4205256
CCTATAC46150.066.04775
GTATAGG153050.065.919626
CGTATAA68900.065.724355
GGTATAG139700.065.6821755
CTATAGG83950.065.6543056
TTATAGG134750.065.340926
CGTATAG86250.064.7973255
GTATAGC99000.064.793386
TATACGG85200.064.445217
CTTATAG72500.064.439855
GGTATAC77350.064.290245
CTATAAC53150.064.129686
GCTATAC67800.063.9518135
TATAGGG154350.063.6872337
AGTATAG108100.063.6780745
TATAGGC106350.063.4090847
GGTATAA100250.063.35835
TTATAGT123250.063.345536
TTATACG79300.063.1494876
Produced by FastQC (version 0.11.5)