FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS30mS1-noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS30mS1-noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7115755
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG97200.065.493516
CTATACG65850.064.732646
TTATAGG162600.064.6133046
CGTATAA85000.064.354815
CCTATAC58050.064.328465
GGTATAG166150.064.160915
CGTATAG99350.064.1127555
CTTATAG87450.064.072625
GTATAGG177850.063.834996
GGTATAC88050.062.8410575
TATAGGG184750.062.6826257
GTATAGC103100.062.4923636
TATACGG96150.062.387337
CTATAAC68350.062.057736
GCTATAC77350.061.9870875
GTTATAA121050.061.9292225
TATAGTG198800.061.861477
AGTATAG125500.061.7132845
TATAGGC117350.061.4652677
GTATAAG135450.061.2868736
Produced by FastQC (version 0.11.5)