FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS30mS1-N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS30mS1-N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5240934
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG57350.066.879736
CCTATAC42250.066.183525
TTATAGG122250.066.127256
GGTATAG131300.066.0756765
CTATAGG77600.065.933026
GTATAGG144450.065.679426
GGTATAC71900.065.467125
CTTATAG70150.065.4539645
CGTATAG85700.065.25685
CGTATAA66200.065.130225
AGTATAG99150.064.946375
TATACGG82050.064.361167
TATAGGG142600.064.346727
GCTATAC63000.064.216575
CCTATAG51100.063.8984875
GTATAGC88800.063.607446
TTATAGT122950.063.5875366
GTTATAG128100.063.5189975
TATAGGC98200.063.4695857
GGTATAA98150.063.326065
Produced by FastQC (version 0.11.5)