FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS20mS3-noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS20mS3-noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11510144
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG146000.065.0543756
CCTATAC97600.064.794945
CGTATAA141700.064.560745
CTATACG106000.064.37966
TTATAGG259700.064.3324366
CGTATAG152850.063.858075
CTTATAG154100.063.635325
GTATAGG288550.063.6248476
GGTATAG266800.063.1470385
TATACGG156700.062.688967
AGTATAG198550.062.555985
TATAGGG284350.062.4597787
GGTATAC147250.062.198685
TATAGTG326900.062.1127057
GTATAGC179900.062.0553256
CTATAAC99800.061.7866866
GCTATAC134700.061.758165
GTTATAG274350.061.7154125
TTATAGT274600.061.631866
GTATAAG205650.061.500546
Produced by FastQC (version 0.11.5)