FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS20mS3-N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS20mS3-N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5093770
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG73250.066.695886
CTATACG57050.066.618586
CGTATAA64600.066.527295
GGTATAG130800.066.3288965
TTATAGG120700.066.10726
CCTATAC47100.066.056395
GTATAGG143150.065.763086
CTTATAG66850.065.387545
CTTATAC63600.064.876995
GGTATAC74800.064.8478555
AGTATAG97250.064.776415
CGTATAG79450.064.752645
TATACGG83650.064.638657
TATAGGG138150.064.268677
TTATACG75800.064.175146
GTTATAG130750.064.105875
GCTATAC62800.064.031615
CTATAAC49750.064.01326
CCTATAG49300.063.9605035
TATAGGC94250.063.9041757
Produced by FastQC (version 0.11.5)