FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS20mS2-noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS20mS2-noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7276827
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG69450.065.759636
CCTATAC59150.065.615115
CTATAGG97100.065.52336
TTATAGG173500.065.010266
GGTATAG169400.064.683985
CGTATAG98700.064.674755
GTATAGG186850.064.617056
CTTATAG91600.064.5681465
CGTATAA86850.064.190845
GGTATAC88550.064.104625
TATACGG104950.063.62437
TATAGGG191450.063.4857837
TATAGGC135350.063.219017
GCTATAC82450.063.1171535
AGTATAG137400.063.03995
GTATAAG127100.063.0262346
GTATAGC107800.062.9477426
GTTATAA122650.062.9455535
TATAGGT180850.062.659287
TTATAGC116850.062.475066
Produced by FastQC (version 0.11.5)