FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS20mS2-N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS20mS2-N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4547188
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG114650.066.361255
CTATAGG64300.066.344166
GTATAGG125000.066.155166
TTATAGG109750.066.0048456
CGTATAA56150.066.004775
CTTATAG58300.065.851735
CTATACG50900.065.727956
CCTATAC40000.065.706615
GGTATAC64200.065.5782245
CGTATAG74300.065.3777165
GTATAGC78700.064.610166
GCTATAC54300.064.515395
AGTATAG85550.064.348415
TATAGGG119950.064.184137
TATACGG71100.064.181997
CTATAAC47750.063.907696
GTATAAG91100.063.8443766
TTATAGT100300.063.745226
TATAGGC87700.063.5255857
CTATACC45850.063.5029566
Produced by FastQC (version 0.11.5)