FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS20mS1-noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS20mS1-noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5476448
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG50850.065.928416
CCTATAC42600.065.883215
CTATAGG75350.065.716026
CGTATAA65750.065.253635
CTTATAG71250.064.931675
CGTATAG73700.064.719975
GTATAGG141100.064.482886
TTATAGG124550.064.369396
GGTATAG131800.063.8571665
GGTATAC74500.063.743165
AGTATAG102700.063.618565
TATACGG78100.063.4468847
TATAGGC98800.063.2590377
GTATAAG98450.063.1995666
GTTATAA93250.063.1604545
GCTATAC62450.063.0980455
CTATAAC50600.062.7962076
TATAGGG139900.062.3845147
GTATAGC87750.062.3717166
GGTATAA98050.062.2813225
Produced by FastQC (version 0.11.5)