FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS20mS1-N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS20mS1-N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7292260
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG185800.065.282875
CTATAGG99550.065.175576
TTATAGG165700.065.091846
CGTATAG112500.064.4856645
CCTATAC57400.064.382585
GTATAGG200150.064.3440866
CTTATAG95900.064.298925
CGTATAA89650.064.17535
CTATACG73550.063.9488876
TTATAGT186100.063.1655126
GTTATAG183150.063.15115
GATATAG124100.063.0827455
GTATAGC130000.062.777156
TATAGGG187600.062.5481077
TATAGGC135950.062.5004127
TATACGG110550.062.4887477
TATAGTG238000.062.462737
GGTATAC96450.062.3357735
TATAGGT194150.062.294517
CTTATAC88600.062.289435
Produced by FastQC (version 0.11.5)