FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS15mS3-noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS15mS3-noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4494879
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC36650.065.979175
CTATAGG59500.065.693826
CTTATAG57300.065.103655
GTATAGG117100.064.99666
CGTATAA53500.064.952855
TTATAGG107350.064.673736
GGTATAG108700.064.4521265
GGTATAC57100.064.228525
CTATACG41200.064.0416266
CGTATAG61450.063.3265275
TATAGGG117000.063.2576077
GCTATAC50800.063.100755
TATAGGC78550.062.725597
TATAAGG85800.062.6458327
GTTATAG105100.062.564335
TATAGTG115450.062.4701167
GTATAAG80700.062.3986936
TATACGG61400.062.122147
GGTATAA77300.062.112275
AGTATAG82500.062.014925
Produced by FastQC (version 0.11.5)