FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS15mS3-N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS15mS3-N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9457883
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG131900.066.672346
TTATAGG211450.066.1657266
GGTATAG231100.066.114335
CCTATAC79300.065.798545
CGTATAA120000.065.558185
CGTATAG142200.064.9950945
CTATACG97250.064.9509666
GTATAGG250650.064.934676
CTTATAG119250.064.914025
TATACGG140000.064.189657
GTATAGC157450.064.016
GGTATAC134650.063.5714155
AGTATAG182000.063.453325
GCTATAC115050.063.4511455
GTTATAG221600.063.3582155
TTATAGT209250.063.3326766
TATAGGC165850.063.1523137
TTATACG131500.063.12296
TATAGGG232000.063.004937
CTATAAC89600.062.8804866
Produced by FastQC (version 0.11.5)