FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS15mS2-noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS15mS2-noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7252696
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC59950.065.5563055
TTATAGG173900.065.181796
CTATACG68900.064.607486
GGTATAG171050.064.550585
CTATAGG95350.064.522786
CTTATAG91700.064.45915
GTATAGG185100.064.376246
CGTATAG98800.064.219185
CGTATAA85300.064.208015
GCTATAC83950.063.447995
TATAGGC126700.063.3619777
GGTATAC92300.063.1300935
GTATAGC114600.063.0289346
TATACGG102250.062.940527
TATAGGG191400.062.8967867
GTTATAA122800.062.7827075
GTATAAG130550.062.7001086
TTATAAG125800.062.0909736
TATAGTG204650.062.056487
TTATAGC118000.061.894976
Produced by FastQC (version 0.11.5)