FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS15mS2-N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS15mS2-N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9361913
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG132650.065.875786
CGTATAA113850.065.874535
GGTATAG225450.065.8023765
TTATAGG223150.065.788446
GTATAGG250900.065.653526
CCTATAC76900.065.397155
CTTATAG118050.065.309575
CTATACG95350.065.256776
CGTATAG135000.064.860745
GGTATAC130550.064.444525
TATACGG139450.064.344877
GCTATAC112150.064.033395
TATAGGG247700.063.972737
TATAGGC177400.063.639277
GTATAGC148350.063.5277186
GGTATAA165600.063.4000055
AGTATAG182250.063.3878975
TATAGTG254400.063.058267
GTATAAG181650.063.0365336
GTTATAA159000.062.840175
Produced by FastQC (version 0.11.5)