FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS15mS1-noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS15mS1-noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5113840
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG71100.066.4950566
GGTATAG120700.065.293995
TTATAGG118550.065.178026
GTATAGG132250.064.9620066
CCTATAC40650.064.911695
CTATACG46050.064.822096
CGTATAG70400.064.721785
CTTATAG65250.064.359535
TATAGGG134500.063.7984737
TATAGGC94750.063.527447
TATACGG72500.063.2332347
CGTATAA57500.063.1744735
GGTATAC62550.063.109385
GTATAGC75150.062.7717936
GTTATAA87150.062.4417535
TATAGTG137700.062.341287
TATACGC52000.062.3188977
GTATAAG96600.062.3095936
TTATAAG92150.062.2805066
GCTATAC60300.062.1561785
Produced by FastQC (version 0.11.5)