FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS15mS1-N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS15mS1-N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8098257
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG118300.066.382036
CCTATAC68650.066.2701345
GGTATAG202050.065.921175
TTATAGG194600.065.818896
CGTATAA102050.065.739155
CTATACG83800.065.689676
CTTATAG107650.065.570265
GTATAGG228900.064.8693546
GGTATAC119550.064.7223365
CGTATAG123750.064.476985
CTATAAC77200.064.369986
TATACGG130400.064.0331957
GTATAAG163300.063.8619846
GCTATAC96250.063.8467525
CCTATAG74300.063.4444775
TTATACG117550.063.4414566
CTTATAC101500.063.4405335
TTATAGT183200.063.381616
TATAGGC160200.063.3501747
GGTATAA150450.063.3388945
Produced by FastQC (version 0.11.5)