FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS120mS5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS120mS5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17603964
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC144450.065.775995
CTTATAG216700.065.5665365
CTATAGG216900.065.440616
GTATAGG418200.065.254156
CGTATAA229000.064.780315
CTATACG167850.064.7364966
TTATAGG397450.064.047146
GCTATAC201700.064.040295
GGTATAG396300.064.0131455
CGTATAG247250.063.608025
TATACGG237100.063.466877
TATAGGG401800.063.2327
GGTATAC233600.062.6957055
TTATAGT420950.062.583246
CTATAAC161600.062.5626566
GTTATAG427650.062.3728525
TATAGGC285200.062.3339167
CTATACC158650.062.2701346
TTATAGC283750.062.2699436
TATAGGT428300.062.212127
Produced by FastQC (version 0.11.5)