FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS120mS3-noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS120mS3-noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10971003
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG147150.065.352856
GGTATAG256800.064.869395
TTATAGG271300.064.753326
GTATAGG288650.064.728646
CCTATAC87250.064.578565
CGTATAA133200.064.265835
CGTATAG143000.064.022065
CTTATAG139100.064.0055855
CTATACG102800.063.9989476
GGTATAC137450.062.94075
TATACGG161050.062.8410877
TATAGGG295600.062.8039677
GCTATAC119250.062.4512525
GTATAGC163350.062.3635986
TTATAAG199800.062.3187266
TATAGGC208350.061.9782037
AGTATAG210900.061.812685
TTATAGC179650.061.789416
TATAGTG305950.061.720247
TATAGGT290600.061.6928527
Produced by FastQC (version 0.11.5)