FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS120mS3-N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS120mS3-N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24440
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATATC252.758492E-670.000016
CCTATAG252.758492E-670.000015
TATACCG453.6379788E-1270.000017
TATAACC500.070.000017
TTATAAT500.070.000016
TTATAAG453.6379788E-1270.000016
GTATAGA252.758492E-670.000016
CGATATA207.7743745E-570.04
CGTTATA309.828727E-870.04
CTATAGG150.002198826870.06
CTATAAC401.2551027E-1070.06
TATACGG353.5142875E-970.07
TATATCG207.7743745E-570.07
ATAAGGC150.002198826870.08
GATATAC353.5142875E-970.05
CACGTAT150.002198826870.03
ATAGGGC150.002198826870.08
TATAACG309.828727E-870.07
ACGTATA401.2551027E-1070.04
ATAACCG150.002198826870.08
Produced by FastQC (version 0.11.5)