FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS120mS2-noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS120mS2-noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6588939
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG85900.065.5088656
TTATAGG157600.065.460376
CTTATAG87300.064.98245
CTATACG60700.064.916786
CCTATAC50800.064.895445
CGTATAA76700.064.700535
GGTATAG152550.064.6710365
GTATAGG165400.064.2140966
CGTATAG90650.064.202425
TATAGGG168050.063.3264477
GTATAGC100900.063.1923376
TATACGG93600.063.036047
TATAGGC114550.062.8720367
CTATAAC58300.062.6670386
TATAGTG180400.062.638127
GCTATAC71300.062.581745
AGTATAG116750.062.469475
GTATAAG119200.062.357046
GGTATAC88000.062.2782025
GTTATAG159250.062.1699455
Produced by FastQC (version 0.11.5)