FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS120mS2-N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS120mS2-N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6812585
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTCGTGAAGGTAATGAAATTATCAAAGCAGCTTGCAAATGGAGTGCTG69910.1026189031035943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG91250.065.7329946
CTTATAG86950.065.122395
CTATACG65350.065.063316
TTATAGG164600.065.0361946
CGTATAA78600.064.7385945
CCTATAC54150.064.692965
GGTATAG155000.064.6640245
GTATAGG170450.064.631356
CGTATAG93400.064.25975
TATACGG99450.063.1985327
TATAGGC125050.063.1896177
GGTATAC91050.063.1892625
TATAGGG174850.063.1057
AGTATAG125350.063.096545
GCTATAC78300.062.8412745
TATAGTG189650.062.7750327
GTATAGC105350.062.5823486
GTATAAG123500.062.4242066
GGTATAA121750.062.2602585
GTTATAA120050.062.0633055
Produced by FastQC (version 0.11.5)