FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS120mS1-noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS120mS1-noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5172074
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC40700.065.5209665
CGTATAG64650.065.012095
CTATAGG65150.064.997236
TTATAGG118150.064.9870456
GTATAGG122250.064.9545446
CTTATAG62100.064.863955
CGTATAA60400.064.603735
GGTATAG113300.064.154385
CTATACG47700.063.9033556
GCTATAC57300.063.6403855
GGTATAC64150.063.445785
TATACGG77050.063.4087567
GTTATAA88150.062.9651955
TATAGGG129150.062.7054757
CTATAAC49450.062.561896
AGTATAG86000.062.4232865
GTATAGC74950.062.2885326
TATAGGT127450.062.1963467
GGTATAA93000.062.127495
TATAGTG134450.062.0557377
Produced by FastQC (version 0.11.5)