FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS120mS1-N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS120mS1-N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12541462
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTCGTGAAGGTAATGAAATTATCAAAGCAGCTTGCAAATGGAGTGCTG138520.11044964295231291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG154800.065.5599446
TTATAGG283600.065.104376
CCTATAC99500.065.034595
GTATAGG304050.064.972636
GGTATAG272950.064.685765
CGTATAG166400.064.672915
CGTATAA144400.064.201535
CTTATAG157550.064.174095
CTATACG121050.063.6307146
TATACGG174850.063.0861247
GGTATAC156800.062.963325
TATAGGG300250.062.9396067
TATAGGC215050.062.440397
GTATAGC187900.062.2989736
TATAGGT307950.062.263627
GCTATAC145800.061.904815
GTTATAG294500.061.877525
TTATAGT306150.061.840726
AGTATAG209200.061.780055
TATAGTG342800.061.7425047
Produced by FastQC (version 0.11.5)