FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS10mS3-noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS10mS3-noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4229112
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG61950.067.054636
CCTATAC32550.065.908145
TTATAGG99750.065.6066746
CGTATAG61800.065.4634555
CTATACG39650.065.314236
CTTATAG54000.065.003515
GGTATAG105150.064.96815
GTATAGG115950.064.8008356
GGTATAC57000.064.652175
CGTATAA52850.064.232725
TATAGGG115900.064.193957
TATACGG62600.063.7860227
GCTATAC48000.063.5047955
TTATAGC70700.063.2602276
GTATAGC71500.062.9929356
TATAGTG122350.062.7833867
TATAGGT117650.062.7036557
TTATAGT100900.062.673846
GGTATAA81600.062.5741165
GTTATAG106750.062.551845
Produced by FastQC (version 0.11.5)