FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS10mS3-N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS10mS3-N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7499614
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG71600.065.786846
TTATAGG168800.065.719446
CTATAGG106800.065.1406566
GTATAGG201550.064.920186
CGTATAA91400.064.784755
CTTATAG97650.064.5446245
CGTATAG110250.064.27845
GGTATAG189800.064.276145
CCTATAC59100.064.188975
TATAGGG193000.063.028677
TATAGGC140300.062.8078277
TATAGTG229800.062.71227
TATACGG109100.062.5818257
GCTATAC87950.062.511365
GGTATAC105150.062.3704535
GTATAAG148250.062.3656046
TATAGGT196700.062.3590357
GTTATAG185750.062.2486345
TTATAGT184850.062.2092746
GTATAGC120400.061.8517726
Produced by FastQC (version 0.11.5)