FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS10mS2-noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS10mS2-noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4031109
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG95650.066.041336
CTATAGG58950.065.421626
CCTATAC32350.065.341885
GGTATAG96750.065.327455
CGTATAA51900.065.206085
CGTATAG56650.064.680855
CTTATAG49400.064.680415
CTATACG37750.064.523196
GTATAGG107850.064.281786
TATAGGG107900.063.6689647
AGTATAG73250.063.4004335
CTATAAC37100.063.2010736
GGTATAC51350.063.1101885
GTATAAG78750.063.104656
TATACGG58900.062.9230237
GGTATAA69050.062.6446155
GTATAGC65900.062.504986
TATAGTG108800.062.4668057
GTTATAG97500.062.4559175
TTATAGT92850.062.454566
Produced by FastQC (version 0.11.5)