FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS10mS2-N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS10mS2-N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9922679
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG143450.066.3067556
TTATAGG237050.066.211196
GGTATAG251250.066.119695
CTATACG105150.065.8968666
CGTATAA125900.065.794595
CTTATAG126900.065.607055
CCTATAC84850.065.496235
CGTATAG153400.064.97295
GGTATAC145950.064.908545
GTATAGG277350.064.855066
GCTATAC119850.064.648325
TATACGG154800.064.451817
CTATAAC96300.064.067086
GTATAGC167850.064.048476
AGTATAG190050.063.8047565
TATAGGG257200.063.7996257
GTTATAG240100.063.7826925
CTTATAC121700.063.723095
TATAGGC187600.063.6107377
TTATAGT224750.063.5908976
Produced by FastQC (version 0.11.5)