FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS10mS1-noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS10mS1-noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2473932
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG34150.066.506456
GGTATAG58550.065.987355
CCTATAC20400.065.703295
GCTATAC27450.065.529875
CGTATAG34150.065.483015
GTATAGG60900.065.2785956
TTATAGG54750.065.0688866
CTTATAG32600.065.053935
CGTATAA29250.064.8473055
CTATACG24050.064.6067056
TATACGG35400.064.552177
TATAGGC45600.064.156757
GGTATAC37000.064.033235
CGCTATA27850.063.4577564
ATTATAG47500.063.2138445
CTATAAC24800.062.9350936
TATAGGG63050.062.884817
TTATAGC42500.062.826866
TATAGGT63300.062.747027
GTATAGC41950.062.5660936
Produced by FastQC (version 0.11.5)