FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS0mS3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS0mS3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8154551
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG118650.066.449636
CGTATAG113700.065.865465
CGTATAA100400.065.772255
GTATAGG210350.065.736
CTATACG83850.065.690236
GGTATAG192550.065.500665
TTATAGG199650.065.484226
CCTATAC66200.065.3907245
CTTATAG106400.065.28655
GGTATAC108500.064.667955
GTATAGC126500.064.096556
TATAGGC149500.064.0901647
TATAGGG217900.063.821917
AGTATAG157150.063.5096325
TATACGG125150.063.4178247
CTATAAC79050.063.3928576
GCTATAC93850.063.1287425
TATAACG92050.062.993817
CGCTATA90400.062.8281944
TATAGCG135550.062.7860577
Produced by FastQC (version 0.11.5)