FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS0mS2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS0mS2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8236932
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG217600.065.809096
GGTATAG205200.065.644075
CTATAGG115600.065.510146
CCTATAC70150.065.503125
CTATACG85250.065.474996
TTATAGG192950.065.383726
CGTATAA102300.065.101075
CTTATAG108950.064.7251055
CGTATAG122050.064.659715
TATAGGG212550.064.0303657
TATACGG123250.063.8574647
GTATAGC137250.063.5141536
AGTATAG152100.063.38955
GCTATAC101800.063.0143785
GGTATAC112150.062.8470885
CGCTATA97900.062.843614
CTATAAC73150.062.766616
TTATAGT199700.062.7180866
TATAGGC149400.062.635417
GTTATAG205700.062.5922365
Produced by FastQC (version 0.11.5)