FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS0mS1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS0mS1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6443922
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG65000.066.32926
CGTATAA77850.066.306745
GGTATAG158200.065.878315
CTATAGG91700.065.5633546
TTATAGG151450.065.507526
CCTATAC54200.065.47315
GTATAGG178200.065.119976
TATACGG96200.064.605867
CTTATAG87950.064.422155
GCTATAC78200.064.130365
GGTATAC91000.064.0320055
CGTATAG99650.064.0226445
AGTATAG123800.063.4629065
TATAGGC123150.063.368657
GTATAAG129300.063.3051576
TATAGGG165350.063.1748437
GTTATAG161100.063.171785
GTATAGC108100.062.932936
TATAGTG188100.062.86427
CTTATAC77950.062.674885
Produced by FastQC (version 0.11.5)