FastQCFastQC Report
Wed 6 Nov 2019
HJ5KLBGXC_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5KLBGXC_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517205326
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14240010.27532605107009284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG17009050.065.169452
AAGCAGT17429100.063.8704571
GCAGTGG18733850.059.1126483
AGTGGTA20068850.055.2978255
CAGTGGT22067300.050.2329834
GTGGTAT22570450.049.178856
GGTATCA22704650.048.818468
TGGTATC23550550.047.084917
GTATCAA23657050.046.7514579
CAACGCA21780100.028.23110612-13
ACGCAGA21893050.028.0928214-15
ATCAACG22387550.027.60153210-11
AGTACAT22584800.027.19787220-21
GCAGAGT22694400.027.19596316-17
AGAGTAC22879650.027.05391118-19
TACATGG22822900.026.6768122-23
CATGGGG13079950.026.61295724-25
GTACATG21516200.025.5837822-23
CGCAGAG22515650.024.7111216-17
TCAACGC22465450.024.6443312-13
Produced by FastQC (version 0.11.5)