FastQCFastQC Report
Wed 6 Nov 2019
HJ5KLBGXC_n02_Ctr2h24hCut_Nov1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5KLBGXC_n02_Ctr2h24hCut_Nov1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145445
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2300.15813537763415725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT5400.067.678611
AGCAGTG5750.063.5590482
GCAGTGG5850.061.746143
AGTGGTA6350.056.215015
GTGGTAT6850.052.7321056
TGGTATC6950.051.9733737
GGTATCA7250.050.4088978
CAGTGGT7000.050.3879624
GTATCAA7250.049.8227469
CAACGCA6400.030.87590612-13
ATCAACG6600.030.5841510-11
ACGCAGA6450.029.64828514-15
AGTACAT6600.028.97445920-21
AGAGTAC6800.028.43473618-19
TACATGG6850.028.22718622-23
GCAGAGT7050.028.02919216-17
CATGGGG3750.027.7639724-25
GTACATG6400.027.22391722-23
TCAACGC6500.026.47819912-13
ATGGGGC907.6215656E-1025.97416726-27
Produced by FastQC (version 0.11.5)