FastQCFastQC Report
Wed 6 Nov 2019
HJ5KLBGXC_n02_24hCut_2h24h_Nov1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5KLBGXC_n02_24hCut_2h24h_Nov1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533006
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27530.5165045046397226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG14400.062.836762
AAGCAGT14950.060.8149031
GCAGTGG15050.060.1228833
AGTGGTA17000.053.97615
CAGTGGT17900.050.5502434
GTGGTAT18800.047.6783526
GGTATCA19000.047.4000638
TGGTATC19150.047.0287867
GTATCAA20600.043.9247259
CAACGCA18550.027.71010412-13
ATCAACG19100.027.24578910-11
ACGCAGA18950.026.79018814-15
AGAGTAC19750.026.35156418-19
TACATGG19650.025.41058222-23
AGTACAT19900.025.29896220-21
GCAGAGT20350.025.05268316-17
CATGGGG11200.024.85556824-25
GTACATG18850.024.3473522-23
CGCAGAG19250.024.05654116-17
TCAACGC18750.024.01603312-13
Produced by FastQC (version 0.11.5)