FastQCFastQC Report
Wed 6 Nov 2019
HJ5KLBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5KLBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517205326
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGG12549010.242631105465453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGGT855300.018.7740023
TCGGCGG855800.018.7647422
ATCGGCG1124550.018.2014711
GCCGATG1264800.017.912571
AACGGGA1011250.017.8742751
TGGGCTG1037400.017.8161411
GGCGGTG606800.017.6808364
TGACGGA719700.017.18074610
GTGCGGG531050.017.104422
CCGGGAG531600.016.941872
ATGGGAG891950.016.9035321
CCGGACA1035150.016.7388041
CTACAGA1753050.016.6543271
CCATGAG1811500.016.5860423
GCGGTGT549000.016.5808475
AGGAACG838400.016.363368
GAAGATG966550.016.3387346
CATCGGG1165650.016.3127861
CAAGGCG626750.016.2641938
CGAGCGA518000.016.109886
Produced by FastQC (version 0.11.5)