FastQCFastQC Report
Wed 6 Nov 2019
HJ5KLBGXC_n01_24hCut_2h24h_Nov1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5KLBGXC_n01_24hCut_2h24h_Nov1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533006
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGG35960.674664075076078No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGAC250.002578563322.011
TGGCACG302.8698603E-422.05
GGGCCCT504.6118657E-822.09
CGTCGGC302.8698603E-422.06
GCGAGCT250.002578563322.05
GGGATAG701.8371793E-1020.4285723
ATGGGAG651.6189006E-920.3076921
GCGGAAA1250.020.241
AATGGCA601.4269972E-820.1666663
AGGAACG601.4269972E-820.1666668
CATCGGG1050.019.9047621
TGACTCC1050.019.9047621
CTCACGC501.121025E-619.86
GTTGCCA501.121025E-619.88
CCCTCGG451.0002381E-519.5555555
TGCGGAG451.0002381E-519.5555553
TACTATG451.0002381E-519.5555552
TGCCGAG451.0002381E-519.5555551
TCGAACG855.456968E-1219.4117648
CGGGTGG408.976563E-519.2500022
Produced by FastQC (version 0.11.5)