FastQCFastQC Report
Mon 14 Nov 2016
HJ5H3BGXY_n01_sl32_08_bt5_bt5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5H3BGXY_n01_sl32_08_bt5_bt5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12866462
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG168630.13106167025558385No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT167580.13024559509832617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT17150.027.34280834
GCTGACT55400.026.5394651
CTAGCAG63000.024.0519228
ACTCTAG65650.023.6169475
GACTCTA66800.023.3152454
GTACGTA21000.022.49668735
CCGTACG21350.022.12763233
TATGCCG21050.021.78292348
CTGACTC70800.021.108522
CGTATGC21800.021.03293646
GTATGCC22150.020.85844447
CTATCGA79500.018.22394417
CTCTAGC87900.017.7582726
TCTATCG82700.017.73036616
TAGCAGA86450.017.7301149
TACGTAA26650.017.5959136
GCACACG28150.017.52884311
TGACTCT91400.017.1931933
GTGCGCA77950.016.52791868
ATGCCGT29800.015.85719149
Produced by FastQC (version 0.11.2)