FastQCFastQC Report
Mon 14 Nov 2016
HJ5H3BGXY_n01_sl32_07_bt5_bt5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5H3BGXY_n01_sl32_07_bt5_bt5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13014734
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG221730.1703684454864771No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC162310.12471249892621701No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT148760.11430122198425262No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA137320.10551118447753138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG23750.025.05448348
CGTATGC23900.024.16468846
GCTGACT46600.023.815441
GTATGCC25700.022.60832847
CTAGCAG57300.019.5431868
ACTCTAG61800.019.0868035
GACTCTA64600.018.801124
ATGCCGT31150.018.4286849
GCACACG34500.017.95426211
AGTCACG33600.017.49719228
CTGACTC65600.017.2341082
TGCCGTC34450.017.1714150
GTGCGCA89000.017.15069668
CTCGTAT35100.016.65346144
TCGTATG35800.016.42566745
GGTGCGC95100.016.050667
GTCACGT37250.015.87664629
TGCGCAT97000.015.77223769
ACGTCTG38050.015.35937315
CTATCGA71800.015.20670817
Produced by FastQC (version 0.11.2)