FastQCFastQC Report
Mon 14 Nov 2016
HJ5H3BGXY_n01_sl32_06_bt5_bt5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5H3BGXY_n01_sl32_06_bt5_bt5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15383245
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG267040.1735914626595364No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC215170.1398729591838393No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT165700.10771459467752091No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA158720.10317719050824453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT56950.023.5430811
CTAGCAG66300.020.5850168
GACTCTA70750.019.7385754
ACTCTAG71500.019.6294925
CTGACTC76300.018.0278682
GTGCGCA107150.017.70961668
GGTGCGC113650.016.81991667
TGCGCAT115250.016.46484269
CTATCGA84100.015.81213717
AGGTGCG121950.015.78936266
CGTATGC23600.015.5729946
TCTATCG85850.015.53072916
CTCTAGC94300.015.4399796
TAGCAGA91550.015.3278739
TATGCCG23100.015.152740548
TCTAGCA150250.014.719787
GTATGCC24800.014.25486147
TGACTCT101300.014.2004813
TAGCATG97500.013.38751927
CCCTACA22600.013.31650210
Produced by FastQC (version 0.11.2)