FastQCFastQC Report
Mon 14 Nov 2016
HJ5H3BGXY_n01_sl32_05_bt5_bt5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5H3BGXY_n01_sl32_05_bt5_bt5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16256950
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG271500.1670054961108941No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC210810.12967377029516608No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA168600.10370949040256629No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT164870.10141508708583098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT61050.020.2428951
CTAGCAG69700.018.877818
ACTCTAG72150.018.385735
GTGCGCA107650.018.0169868
GACTCTA75200.017.7329584
GGTGCGC114350.017.1759667
TATGCCG23650.016.72466548
TGCGCAT116400.016.7226169
AGGTGCG121700.016.10931466
CGTATGC23600.016.01815646
CTGACTC79900.015.8138822
GTATGCC26450.014.68919847
CTCTAGC95400.014.6384766
TCTATCG87350.014.30247516
CTATCGA88450.014.00578217
TAGCAGA95050.013.9534749
TCTAGCA152850.013.8741517
AGCGCGC66050.013.35707461
AAGGTGC151450.013.17580565
GCGCGCC70400.012.73085562
Produced by FastQC (version 0.11.2)