FastQCFastQC Report
Mon 14 Nov 2016
HJ5H3BGXY_n01_sl32_04_ev_bt5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5H3BGXY_n01_sl32_04_ev_bt5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16676760
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG248340.1489138177919452No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC183840.11023724032725782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT58850.018.9762861
CTAGCAG63900.018.1819928
GTGCGCA101400.017.8506868
GACTCTA72950.017.7526284
ACTCTAG71200.017.451685
GGTGCGC109700.016.59582767
TGCGCAT110500.016.28552469
AGGTGCG116500.015.53661466
CGTCCGC20200.015.07172633
CTGACTC78500.014.6692722
GTCCGCA23350.013.78783334
TCTAGCA139400.013.5309287
CGTATGC24850.013.38088346
CTCTAGC92150.013.3320046
TCCGCAC23850.012.91183835
CTATCGA87950.012.69273317
TCTATCG87950.012.61330516
TAGCAGA92400.012.4602159
TATGCCG25300.012.45145148
AAGGTGC146900.012.4399465
Produced by FastQC (version 0.11.2)