FastQCFastQC Report
Mon 14 Nov 2016
HJ5H3BGXY_n01_sl32_03_ev_bt5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5H3BGXY_n01_sl32_03_ev_bt5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15673918
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG209870.13389759982156343No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC160390.10232923255053396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT55650.017.929281
CCCGTCC21050.017.78762833
ACTCTAG64700.016.9864185
TATGCCG22100.016.94662346
GACTCTA65100.016.8821014
CTAGCAG60300.016.8292858
GTGCGCA85950.016.2531168
GTATGCC24700.015.8714145
CGTATGC24350.015.52450844
GGTGCGC92950.015.06700767
CACCCGT25600.015.03623631
TGCGCAT94700.014.67738369
AGGTGCG99750.013.96905966
CCCTACA22950.013.87556310
CTGACTC73850.013.5550242
TCTAGCA128750.013.1275877
CTCTAGC83350.013.0175246
TCCCTAC25700.012.5267479
TCACCCG30800.012.27045830
AGCGCGC58050.012.12211461
Produced by FastQC (version 0.11.2)