FastQCFastQC Report
Mon 14 Nov 2016
HJ5H3BGXY_n01_sl32_02_ev_bt5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5H3BGXY_n01_sl32_02_ev_bt5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17547128
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG239360.1364097874022461No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC183050.10431906577532232No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT176650.1006717452565457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT68200.019.1468831
GTGCGCA101200.017.95586468
CTAGCAG76050.017.8533298
TATGCCG26450.017.60121548
GACTCTA81500.017.3496574
ACTCTAG79100.017.256655
CGTATGC26800.017.2399546
GTATGCC27250.016.95525447
GGTGCGC109050.016.95255367
TGCGCAT110250.016.5452669
AGGTGCG118050.015.48153766
CCCTACA24500.014.9973210
CTGACTC92400.014.7728232
TAGCAGA100600.013.5311099
CTATCGA96100.013.436686517
CTCTAGC105050.013.3268836
ATGCCGT36200.012.95747449
TCCCTAC29750.012.9386059
TCTATCG99850.012.93223816
TCTAGCA166350.012.7688027
Produced by FastQC (version 0.11.2)