FastQCFastQC Report
Mon 14 Nov 2016
HJ5H3BGXY_n01_sl31_12_bzip11_bzip11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5H3BGXY_n01_sl31_12_bzip11_bzip11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16314896
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG12200.015.4952811
CCGGGGT11700.012.2677111
GCGGGGG14350.012.197861
GGATAAC104650.011.4741471
CGGGGGT13650.011.2845841
CGGGCAT18650.011.2625831
CCCTACA20150.010.94105710
GGGGTAT13600.010.811251
GGGGGGC7151.8189894E-1210.7691063
CGGGGAG24350.09.9200921
GGCCATC108600.09.86019210
GGGCGCC62850.09.46998266
CATGCGC109750.09.37393628
CGGCAAT32150.09.3644671
TCATGCG112450.09.33557227
GGGGCAC8252.3646862E-119.3354291
GGCCACG112250.09.3255265
GCGGGCT15800.09.3058861
ACATGGC122050.09.26254756
ATGCGCT112650.09.25687129
Produced by FastQC (version 0.11.2)