FastQCFastQC Report
Mon 14 Nov 2016
HJ5H3BGXY_n01_sl31_11_bzip11_bzip11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5H3BGXY_n01_sl31_11_bzip11_bzip11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11914808
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG427640.3589147219157875No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA262790.22055747772016135No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC233510.1959830154208108No Hit
CGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTG201260.168915856638227No Hit
CTTGACCTCAGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCTGCT200170.16800102863596292No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACA199750.16764852610298042No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG186830.15680487675504298No Hit
GGTACATCCGCTCGGAGGAGGCCTCCCAGCCCATGGTCTTCTTCTGCATT162360.1362674077500871No Hit
CCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTG160450.134664360516762No Hit
GGCCACCCTTGGTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGG150790.1265568022581648No Hit
CTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACT147700.12396339076550793No Hit
TTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCC142830.11987603996640146No Hit
GCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGT141070.1183988864948558No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG129980.10909114104062777No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACATGAAC122730.10300627588795389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG7150.022.52081
CGCGGGG7650.021.0488551
CGGGGGT6300.019.447311
GGGCGCC76800.019.10227666
GGATAAC165600.018.9822651
AGGGCGC78150.018.9505565
CAGGGCG83250.017.62139364
CCGGGGT7900.017.2810291
ATAACAT185050.016.5861633
CGCCCTT90700.016.36596557
GATAACA195500.016.2545342
TCAGGGC93350.015.82762963
GCGCCGT97300.015.61774468
GGGGGGC5500.015.271582
GCCAAAT57300.015.1505741
CGGGGCT13750.014.7658121
TAACATG210250.014.7312934
ACATGGC213900.014.6761936
CGGGCAT12800.014.4943221
CGGGCCT7250.014.4848931
Produced by FastQC (version 0.11.2)